CD68 antibody (PE)

Catalog No. ABIN181838

The Rat Monoclonal anti-CD68 antibody has been validated for FACS. It is suitable to detect CD68 in samples from Mouse. There is 1 publication available.

Quick Overview for CD68 antibody (PE) (ABIN181838)

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD68 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: FA-11

Product Details anti-CD68 Antibody (PE)

Specificity: The antibody recognizes CD68. SM1550R does detect surface Macrosialin at low levels in resident Mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.

Purification: Affinity Chromatography on Protein G

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL. Membrane permeabilisation is required for this application. ABIN181838 does detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water.

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-CD68 antibody (PE) (ABIN181838)

Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68

Background: Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.Synonyms: Gp110, Macrophage marker, Macrosialin

Gene ID: 12514

NCBI Accession: NP_033983

UniProt: P31996